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3-morpholinopropanesulfonic acid hemisodium gishiri CAS: 117961-20-3

3-(N-Morpholino)propanesulfonic acid hemisodium gishiri, wanda kuma aka sani da MOPS-Na, wani buffer ne na zwitterionic wanda aka saba amfani dashi a cikin binciken kwayoyin halitta da nazarin halittu.Ya ƙunshi zoben morpholine, sarkar propane, da ƙungiyar sulfonic acid.

MOPS-Na yana da tasiri mai tasiri don kiyaye tsayayyen pH a cikin kewayon ilimin lissafi (pH 6.5-7.9).Ana amfani da shi sau da yawa a cikin kafofin watsa labarai na al'adar tantanin halitta, tsarkakewar furotin da haɓakawa, ƙididdigar enzyme, da DNA/RNA electrophoresis.

Ɗaya daga cikin fa'idodin MOPS-Na a matsayin mai ɗaukar hoto shine ƙarancin ɗaukar UV, wanda ya sa ya dace da aikace-aikacen spectrophotometric.Hakanan yana nuna ƙaramin tsangwama tare da hanyoyin tantancewa na gama gari.

MOPS-Na yana narkewa a cikin ruwa, kuma narkewar sa yana dogara da pH.Yawancin lokaci ana ba da shi azaman foda mai ƙarfi ko azaman bayani, tare da nau'in gishirin hemisodium da aka fi amfani dashi.


Cikakken Bayani

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Aikace-aikace da Tasiri

Buffering pH: MOPS-Na yana da tasiri a kiyaye tsayayyen pH a cikin kewayon ilimin lissafi (pH 6.5-7.9).Halinsa na zwitterionic yana ba shi damar tsayayya da canje-canje a cikin pH lokacin da aka ƙara acid ko tushe, yana sa ya zama mai amfani don kiyaye yanayi mafi kyau a cikin kafofin watsa labaru na al'adun tantanin halitta da kuma tsarin gwaji daban-daban.

Protein da nazarin enzyme: MOPS-Na ana amfani dashi sau da yawa a cikin tsarkakewar furotin, haɓakawa, da daidaitawa.Ƙarfin buffersa da dacewa tare da yawancin enzymes da sunadarai sun sa ya dace don kiyaye pH da ake so yayin waɗannan matakai.Hakanan za'a iya amfani da MOPS-Na a cikin gwaje-gwajen enzyme, inda madaidaicin kulawar pH ke da mahimmanci don ingantaccen auna ayyukan enzymatic.

DNA da RNA electrophoresis: MOPS-Na yawanci ana amfani dashi azaman mai ɗaukar hoto a cikin gel electrophoresis na nucleic acid.Yana ba da kewayon pH da ake so da ƙarfin ionic, yana ba da damar ingantaccen rabuwar DNA da gutsuttsuran RNA.Ƙananan shayarwar UV na MOPS-Na yana da fa'ida a cikin wannan aikace-aikacen, saboda baya tsoma baki tare da ma'auni na spectrophotometric na nucleic acid.

Kafofin watsa labarai na al'adar salula: Ana amfani da MOPS-Na a cikin kafofin watsa labarai na al'ada don kiyaye pH da ma'aunin osmotic da ake buƙata don haɓakar tantanin halitta da yuwuwa.Daidaituwarta tare da nau'ikan tantanin halitta daban-daban da yanayin marasa guba sun sa ya zama madaidaicin ma'anar buffer don kiyaye yanayin ilimin lissafi a cikin gwaje-gwajen al'adun tantanin halitta.

Pharmacological da nazarin halittu: MOPS-Na yana aiki a cikin nau'o'in ilimin harhada magunguna da nazarin halittu, kamar ma'auni na kinetics na enzyme, ƙididdigar gwajin magani, da kuma nazarin da ke tattare da tasirin pH akan hanyoyin salula.Ƙarfin buffer ɗin sa yana taimakawa rage yawan kayan tarihi masu dogaro da pH da kuma tabbatar da ingantaccen sakamako mai iya sakewa.

 

Shirya samfur:

6892-68-8-3

Ƙarin Bayani:

Abun ciki Saukewa: C7H16NNAO4S
Assay 99%
Bayyanar Farin foda
CAS No. 117961-20-3
Shiryawa Karami da girma
Rayuwar Rayuwa shekaru 2
Adana Ajiye a wuri mai sanyi da bushewa
Takaddun shaida ISO.

 


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